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heresacorrection t1_iy1ez3m wrote on November 28, 2022 at 12:34 AM

Reply to comment by New_Concert_4315 in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918

The “cap” only allows 3’ additions OP probably simplified the wording it’s not an actual 5’ cap (as you hear about in mRNA), it’s a chemical blocking done by modifying the 3’ end of the chain. The 5’ is never exposed.